Cat | 338-APA056Hu61 |
Buy Price | 134 |
Predicted Molecular Mass(KD) | 20.3kDa |
Accurate Molecular Mass(KD) | 18kDa |
Organism species | Homo sapiens (Human) |
Purity | > 97% |
Sequence info | Ser19~Asn178 |
Tag | N-terminal His-Tag |
Isoelectric Point | 7.6 |
Host | 293F cell |
Subcellular Location | Secreted |
Endotoxin Level | Eukaryotic expression |
Source | 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300. |
Buffer Formulation | Freeze-dried powder |
Traits | 335 |
Application | Interleukin 10 |
Item Name | Cytokine;Infection immunity; |
Research Area | CSIF; IL10A; TGIF; Cytokine Synthesis Inhibitory Factor |
Alternative Names | http://www.cloud-clone.com/manual/Active-Interleukin-10-(IL10)-APA056Hu61.pdf |
Reference | http://static.cloud-clone.com/product/pictures/APA056Hu61-201606171655574894.jpg |
Datasheet | Interleukin 10 (IL-10), also known as human cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine. IL-10 was initially reported to suppress cytokine secretion, antigen presentation and CD4 T cell activation. Further investigation has shown that IL-10 predominantly inhibits lipopolysaccharide (LPS) mediated induction of the pro-inflammatory cytokines TNFα, IL-1β, IL-12, and IFNγ made by cells such as macrophages and Th1 T cells. Therefore, THP1 cells were cultured in 24 well plates at a concentration of 106 cells/ml and activated by LPS (1ug/mL) in the absence or presence of IL-10 (5ng/mL, 10ng/mL) for 24h and production of IL-1β was determined in the supernatants by cytokine specific ELISA.<br/>Activation of THP1 cells with LPS (1ug/mL) resulted in high levels of production of IL-1β and strong inhibitory effects of IL-10 (5ng/mL, 10ng/mL) were observed on the production of IL-1β as shown in Table 1. |
Image 1 | http://static.cloud-clone.com/product/od/APA056Hu61.jpg |
provider | 5db5d9fc8a85522c2e67c9ea |
isPharmaceutique | |