Active Tumor Necrosis Factor Receptor Superfamily Member 12A TNFRSF12A

Product NameActive Tumor Necrosis Factor Receptor Superfamily Member 12A TNFRSF12A
Cat338-APA813Hu01
Buy Price140.8
Predicted Molecular Mass(KD)40.8kDa
Accurate Molecular Mass(KD)44kDa
Organism speciesHomo sapiens (Human)
Purity> 80%
Sequence infoSer24~Ala126
TagN-terminal His and GST-Tag
Isoelectric Point6.7
HostE.coli
Subcellular LocationMembrane; Single-pass type I membrane prote
Endotoxin LevelProkaryotic expression
Source20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Buffer FormulationFreeze-dried powder
Traits352
ApplicationTumor Necrosis Factor Receptor Superfamily, Member 12A
Item NameCD & Adhesion molecule;Tumor immunity;
Research AreaCD266; TNFRSF12-A; FN14; TWEAKR; Tumour Necrosis Factor Related Weak Inducer Of Apoptosis; Fibroblast growth factor-inducible immediate-early response protein 14
Alternative Nameshttp://www.cloud-clone.com/manual/Active-Tumor-Necrosis-Factor-Receptor-Superfamily--Member-12A-(TNFRSF12A)-APA813Hu01.pdf
Referencehttp://static.cloud-clone.com/product/pictures/APA813Hu01-201703071036053962.jpg
DatasheetTNFRSF12A (Tumor necrosis factor receptor superfamily member 12A) belongs to the Tumor necrosis factor receptor superfamily. TNFRSF12A is thought to induce apoptosis weakly in some cell types and promotes angiogenesis and the proliferation of endothelial cells. A binding ELISA assay was conducted to detect the association of TNFRSF12A with TNFa. Briefly, TNFRSF12A were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL TNFRSF12A were then transferred to TNFa-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-TNFRSF12A pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of TNFRSF12A and TNFa was shown in Figure 1, and this effect was in a dose dependent manner.
Image 1http://static.cloud-clone.com/product/od/APA813Hu01.jpg
provider5db5d9fc8a85522c2e67c9ea
isPharmaceutique
10 ug
354 £
50 ug
660 £
100 ug
967 £
200 ug
117 £
500 ug
219 £
1 mg
321 £
5 mg
781 £