Active Interleukin 10 IL10

Product NameActive Interleukin 10 IL10
Cat338-APA056Hu61
Buy Price134
Predicted Molecular Mass(KD)20.3kDa
Accurate Molecular Mass(KD)18kDa
Organism speciesHomo sapiens (Human)
Purity> 97%
Sequence infoSer19~Asn178
TagN-terminal His-Tag
Isoelectric Point7.6
Host293F cell
Subcellular LocationSecreted
Endotoxin LevelEukaryotic expression
Source20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Buffer FormulationFreeze-dried powder
Traits335
ApplicationInterleukin 10
Item NameCytokine;Infection immunity;
Research AreaCSIF; IL10A; TGIF; Cytokine Synthesis Inhibitory Factor
Alternative Nameshttp://www.cloud-clone.com/manual/Active-Interleukin-10-(IL10)-APA056Hu61.pdf
Referencehttp://static.cloud-clone.com/product/pictures/APA056Hu61-201606171655574894.jpg
DatasheetInterleukin 10 (IL-10), also known as human cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine. IL-10 was initially reported to suppress cytokine secretion, antigen presentation and CD4 T cell activation. Further investigation has shown that IL-10 predominantly inhibits lipopolysaccharide (LPS) mediated induction of the pro-inflammatory cytokines TNFα, IL-1β, IL-12, and IFNγ made by cells such as macrophages and Th1 T cells. Therefore, THP1 cells were cultured in 24 well plates at a concentration of 106 cells/ml and activated by LPS (1ug/mL) in the absence or presence of IL-10 (5ng/mL, 10ng/mL) for 24h and production of IL-1β was determined in the supernatants by cytokine specific ELISA.<br/>Activation of THP1 cells with LPS (1ug/mL) resulted in high levels of production of IL-1β and strong inhibitory effects of IL-10 (5ng/mL, 10ng/mL) were observed on the production of IL-1β as shown in Table 1.
Image 1http://static.cloud-clone.com/product/od/APA056Hu61.jpg
provider5db5d9fc8a85522c2e67c9ea
isPharmaceutique
10 ug
344 £
50 ug
635 £
100 ug
927 £
200 ug
112 £
500 ug
209 £
1 mg
306 £
5 mg
743 £